This study investigates the connection between mindfulness and prenatal testosterone exposure and explores whether this is related to the relationship between mindfulness and human well-being as captured by three separate measures. In a sample of 90 German student participants, we find that subjects’ digit ratio – a reliable indicator for exposure to prenatal testosterone – predicts their Mindful Attention Awareness Scale (MAAS) scores. Respondents with moderate levels of testosterone exposure have the highest MAAS scores. We additionally elicit participants’ self-reported general life satisfaction and current happiness levels as well as their estimates about others’ general life satisfaction. We find that MAAS strongly predicts absolute and relative life satisfaction and also current happiness levels, but digit ratios do not mediate the relationship between human well-being and mindfulness.